@article { ISI:000265921000002,
	title = {Purification and characterization of putative alkaline [Ni-Fe] hydrogenase from unicellular marine green alga, tetraselmis kochinensis NCIM 1605},
	journal = {Microbiological Research},
	volume = {164},
	number = {2},
	year = {2009},
	month = {FEB},
	pages = {131-137},
	publisher = {ELSEVIER GMBH, URBAN \& FISCHER VERLAG},
	address = {OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY},
	abstract = {<p>Hydrogenase enzyme from the unicellular marine green alga Tetraselmis kochinensis NCIM 1605 was purified 467 fold to homogeneity. The molecular weight was estimated to be similar to 89kDa by SDS-PAGE. This enzyme consists of two subunits with molecular masses of similar to 70 and similar to 19 kDa. The hydrogenase was found to contain 10 g atoms of Fe and 1 g of atom of Ni per mote of protein. The specific activity of hydrogen evolution was 50 mu mol H-2/mg/h of enzyme using reduced methyl viologen as an electron donor. This hydrogenase enzyme has pl value similar to 9.6 representing its alkaline nature. The absorption spectrum of the hydrogenase enzyme showed an absorption peak at 425 nm indicating that the enzyme had iron-sulfur clusters. The total of 16 cysteine residues were found per mote of enzyme under the denaturing condition and 20 cysteine residues in reduced denatured enzyme indicating that it has two disulfide bridges. (C) 2007 Published by Elsevier GmbH.</p>},
	keywords = {Characterization, Chemical modification, Ni-Fe hydrogenase, Tetraselmis kochinensis, Unicellular green algae},
	issn = {0944-5013},
	doi = {10.1016/j.micres.2006.11.006},
	author = {Bhosale, Siddharth H. and Pant, A. and Khan, Mohammad Islam}
}
@article { ISI:000305767200035,
	title = {Intracellular synthesis of gold nanoparticles using alga tetraselmis kochinensis},
	journal = {Materials Letters},
	volume = {79},
	year = {2012},
	month = {JUL},
	pages = {116-118},
	publisher = {ELSEVIER SCIENCE BV},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>The development of eco-friendly synthesis protocol for the synthesis of nanoscale materials of different compositions, shapes and sizes is an important area of research in the field of nanotechnology. In this paper, we report on the use of alga Tetraselmis kochinensis in the intracellular synthesis of gold nanoparticles of dimensions 5-35 nm. The particles are more concentrated upon the cell wall than on the cytoplasmic membrane, possibly due to reduction of the metal ions by enzymes present in the cell wall and cytoplasmic membrane. These intracellular nanoparticles may have applications in drug delivery, biomedical applications, catalysis, etc. (C) 2012 Elsevier B.V. All rights reserved.</p>},
	keywords = {Algae, Biosynthesis, Nanoparticles, nanotechnology, Tetraselmis kochinensis},
	issn = {0167-577X},
	doi = {10.1016/j.matlet.2012.04.009},
	author = {Senapati, Satyajyoti and Syed, Asad and Moeez, Sana and Kumar, Ashutosh and Ahmad, Absar}
}
