01029nas a2200157   4500008004100000245008800041210006900129260000800198300001400206490000600220520046600226100002400692700001900716700002100735856011500756       2016                        eng d00aDraft genome sequence of arthrobacter enclensis NCIM 5488T for secondary metabolism0 aDraft genome sequence of arthrobacter enclensis NCIM 5488T for s  cJUN  ae00497-160 v43 aHere, we report the draft genome sequence of Arthrobacter enclensis NCIM 5488T, an actinobacterium isolated from a marine sediment sample from Chorao Island, Goa, India. This draft genome sequence consists of 4,226,231 bp with a G+C content of 67.08%, 3,888 protein-coding genes, 50 tRNAs, and 10 rRNAs. Analysis of the genome using bioinformatics tools such as antiSMASH and NaPDoS showed the presence of many unique natural product biosynthetic gene clusters.1 aNeurgaonkar, P., S.1 aDharne, M., S.1 aDastager, S., G.  uhttp://library.ncl.res.in/content/draft-genome-sequence-arthrobacter-enclensis-ncim-5488t-secondary-metabolism02574nas a2200181   4500008004100000245013100041210006900172260000800241300001100249490000600260520190300266100001802169700002402187700001702211700002102228700001902249856012402268       2016                        eng d00aPeeping into genomic architecture by re-sequencing of Ochrobactrum intermedium M86 strain during laboratory adapted conditions0 aPeeping into genomic architecture by resequencing of Ochrobactru  cJUN  a 72-760 v83 aAdvances in de novo sequencing technologies allow us to track deeper insights into microbial genomes for restructuring events during the course of their evolution inside and outside the host. Bacterial species belonging to Ochrobactrum genus are being reported as emerging, and opportunistic pathogens in this technology driven era probably due to insertion and deletion of genes. The Ochrobactrum intermedium M86 was isolated in 2005 from a case of non-ulcer dyspeptic human stomach followed by its first draft genome sequence in 2009. Here we report re-sequencing of O. intermedium M86 laboratory adapted strain in terms of gain and loss of genes. We also attempted for finer scale genome sequence with 10 times more genome coverage than earlier one followed by comparative evaluation on Ion PGM and Illumina MiSeq. Despite their similarities at genomic level, lab-adapted strain mainly lacked genes encoding for transposase protein, insertion elements family, phage tail-proteins that were not detected in original strain on both chromosomes. Interestingly, a 5 kb indel was detected in chromosome 2 that was absent in original strain mapped with phage integrase gene of Rhizobium spp. and may be acquired and integrated through horizontal gene transfer indicating the gene loss and gene gain phenomenon in this genus. Majority of indel fragments did not match with known genes indicating more bioinformatic dissection of this fragment. Additionally we report genes related to antibiotic resistance, heavy metal tolerance in earlier and re-sequenced strain. Though SNPs detected, there did not span urease and flagellar genes. We also conclude that third generation sequencing technologies might be useful for understanding genomic architecture and re-arrangement of genes in the genome due to their ability of larger coverage that can be used to trace evolutionary aspects in microbial system. 1 aGohil, K., N.1 aNeurgaonkar, P., S.1 aParanjpe, A.1 aDastager, S., G.1 aDharne, M., S.  uhttp://library.ncl.res.in/content/peeping-genomic-architecture-re-sequencing-ochrobactrum-intermedium-m86-strain-during