02034nas a2200241 4500008004100000245012600041210006900167260000800236300000800244490000700252520115000259653002501409653002901434653002701463653003101490653001401521653002801535100002701563700002101590700002101611700003001632856013001662 2022 eng d00aAssembly, annotation, and comparative whole genome sequence of fusarium verticillioides isolated from stored maize grains0 aAssembly annotation and comparative whole genome sequence of fus cJUL a8100 v113 a

Fusarium verticillioides is a plant pathogenic fungus affecting a wide range of crops worldwide due to its toxigenic properties. F. verticillioides BIONCL4 strain was isolated from stored maize grain samples in India, and produces high amount of fumonisin B1 (FB1). We report a comparative genomic analysis of F. verticillioides, covering the basic genome information, secretome, and proteins involved in host-pathogen interactions and mycotoxin biosynthesis. Whole-genome sequencing (WGS) was performed using the Illumina platform with an assembly size of 42.91 Mb, GC content of 48.24%, and 98.50% coverage with the reference genome (GCA000149555). It encodes 15,053 proteins, including 2058 secretory proteins, 676 classical secretory proteins, and 569 virulence and pathogenicity-related proteins. There were also 1447 genes linked to carbohydrate active enzymes (CaZymes) and 167 genes related to mycotoxin production. Furthermore, F. verticillioides genome comparison revealed information about the species' evolutionary history. The overall study helps in disease prevention and management of mycotoxins to ensure food safety.

10acomparative genomics10aFusarium verticillioides10amycotoxin biosynthesis10aplant-pathogen interaction10aSecretome10awhole-genome sequencing1 aNavale, Vishwambar, D.1 aSawant, Amol, M.1 aGowda, Varun, U.1 aVamkudoth, Koteswara, Rao uhttp://library.ncl.res.in/content/assembly-annotation-and-comparative-whole-genome-sequence-fusarium-verticillioides-isolated02467nas a2200229 4500008004100000022001400041245009600055210006900151260000800220300001400228490000700242520169800249653001401947653001601961653002901977653001402006653001802020100002702038700002102065700003002086856012102116 2023 eng d a1415-475700aGenetic diversity of toxigenic Fusarium verticillioides associated with maize grains, India0 aGenetic diversity of toxigenic Fusarium verticillioides associat cMAY ae202200730 v463 a

In the present investigation, prevalence, genetic diversity, and mycotoxin producing potential of Fusarium species associated with maize grain samples were studied from different geographical regions of India. The highest prevalence of Fusarium verticillioides was recorded as 88.52%, followed by F. coffeatum, F. foetens, and F. euwallaceae, 6.55%, 3.27%, and 1.63%, respectively. We isolated 54 strains of F. verticillioides, and their genetic diversity was studied by inter simple sequence repeats (ISSR). The ISSR fingerprints (AG) 8C and (AG) 8G showed 252 and 368 microsatellite sites in the genome of F. verticillioides and resulted in 99-100% repeatability and reproducibility. The Simpson (SID) and Shannon (H) indices (0.78 and 2.36) suggest that F. verticillioides strains exhibit moderate to high diversity. Molecular detection of fumonisin B1 (FB1) biosynthetic genes (FUM1 and FUM13) involved in FB1 production in F. verticillioides was confirmed by polymerase chain reaction (PCR). Furthermore, 91% of the strains were positive for FB1 production, which was affirmed by liquid chromatography with tandem mass spectrometry (LC-MS-MS). In-vitro appurtenance of F. verticillioides spores exhibited a high to moderate effect on the growth and development of the maize. The current finding demonstrated that most F. verticillioides strains showed a wide range of genetic diversity with varied toxigenic and pathogenic potentials. In conclusion, for the first time, F. coffeatum, F. foetens, and F. euwallaceae species were reported from maize grain samples in India. They were positive for FB1 and negatively affecting grain quality, which is a major concern in food safety.

10adiversity10aFood safety10aFusarium verticillioides10aMycotoxin10apathogenicity1 aNavale, Vishwambar, D.1 aSawant, Amol, M.1 aVamkudoth, Koteswara, Rao uhttp://library.ncl.res.in/content/genetic-diversity-toxigenic-fusarium-verticillioides-associated-maize-grains-india02727nas a2200241 4500008004100000022001400041245013800055210006900193260000800262300001100270490000800281520187500289653002802164653001602192653001802208653002902226653001102255653003002266653001002296100002702306700003002333856012202363 2025 eng d a0956-713500aUnlocking the potential of lactococcus lactis subsp. lactis BIONCL17752 strain on fumonisin B1 production by fusarium verticillioides0 aUnlocking the potential of lactococcus lactis subsp lactis BIONC cFEB a1109100 v1683 a

Mycotoxins are the potent toxic chemical agent's linked to food safety, pose a serious risk to human and animal health globally. The management of toxigenic fungi is a challenging task in food and feed industries. The present investigation aimed to use probiotic potentials of lactic acid bacteria (LAB), and fermented cell-free broth as antifungal agents and neutralization of carcinogenic mycotoxin, fumonisin B1 (FB1) in food and feed considered as ``turned waste into treasure''. We strived to investigate the probiotic potential of Lactococcus lactis subsp. lactis BIONCL17752 strain on prevention of growth and neutralization of FB1 produced by Fusarium verticillioides BIONCL4 strain to emulate as as a food grade bio-preservative. The BIONCL17752 strain exhibited excellent inhibitory activity against BIONCL4 strain. It was cultivated in MRS medium, and obtained cells pellet (CP) and cell-free supernatant (CFS) was lyophilized, and used for their antifungal activity against BIONCL4 strain. The minimum fungicidal concentration (MFC) determined as 12.5 mu g/mL. Nevertheless, CFS assessed for neutralization of FB1 production which exhibited complete inhibition and downregulation of FB1 encoding FUM1 gene expression using quantitative real-time PCR (qPCR). Furthermore, CFS induced transcriptomic studies against BIONCL4 strain endorsed a significant downregulation of virulence, FB1, fusaric acid, fusarin, and chitin biosynthetic pathway genes. The CFS and cells reticent spore germination and FB1 production in the range of 40-61% and 74-85%, respectively under stored maize for 60 days. The current findings suggest BIONCL17752 strain apprehend the fungal growth and inhibit the FB1, and other toxigenic molecules biosynthesis, and can be employed as food grade preservative in the food industries to ensure the food safety and human health.

10aFood grade preservative10aFood safety10aFumonisin B-110aFusarium verticillioides10alactis10aLactococcus lactis subsp.10aMaize1 aNavale, Vishwambar, D.1 aVamkudoth, Koteswara, Rao uhttp://library.ncl.res.in/content/unlocking-potential-lactococcus-lactis-subsp-lactis-bioncl17752-strain-fumonisin-b1