03891nas a2200337 4500008004100000022001400041245018200055210006900237260008400306300001400390490000600404520267800410653001203088653001003100653001003110653003803120653001403158653001703172100002503189700001903214700002603233700002003259700002003279700002103299700002003320700001803340700001703358700002403375700002603399856012803425 2014 eng d a1178-201300aRapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant plumbago zeylanica and their application in biofilm control0 aRapid efficient synthesis and characterization of silver gold an aPO BOX 300-008, ALBANY, AUCKLAND 0752, NEW ZEALANDbDOVE MEDICAL PRESS LTDcMAY a2635-26530 v93 a

Background: Nanoparticles (NPs) have gained significance in medical fields due to their high surface-area-to-volume ratio. In this study, we synthesized NPs from a medicinally important plant - Plumbago zeylanica. Materials and methods: Aqueous root extract of P. zeylanica (PZRE) was analyzed for the presence of flavonoids, sugars, and organic acids using high-performance thin-layer chromatography (HPTLC), gas chromatography-time of flight-mass spectrometry (GC-TOF-MS), and biochemical methods. The silver NPs (AgNPs), gold NPs (AuNPs), and bimetallic NPs (AgAuNPs) were synthesized from root extract and characterized using ultraviolet-visible spectra, X-ray diffraction (XRD), energy-dispersive spectrometry (EDS), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The effects of these NPs on Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, scanning electron microscopy, and atomic force microscopy. Results: PZRE showed the presence of phenolics, such as plumbagin, and flavonoids, in addition to citric acid, sucrose, glucose, fructose, and starch, using HPTLC, GC-TOF-MS, and quantitative analysis. Bioreduction of silver nitrate (AgNO3) and chloroauric acid (HAuCl4) were confirmed at absorbances of 440 nm (AgNPs), 570 nm (AuNPs), and 540 nm (AgAuNPs), respectively. The maximum rate of synthesis at 50 degrees C was achieved with 5 mM AgNO3 within 4.5 hours for AgNPs; and with 0.7 mM HAuCl4 within 5 hours for AuNPs. The synthesis of AgAuNPs, which completed within 90 minutes with 0.7 mM AgNO3 and HAuCl4, was found to be the fastest. Fourier-transform infrared spectroscopy confirmed bioreduction, while EDS and XRD patterns confirmed purity and the crystalline nature of the NPs, respectively. TEM micrographs and DLS showed about 60 nm monodispersed Ag nanospheres, 20-30 nm Au nanospheres adhering to form Au nanotriangles, and about 90 nm hexagonal blunt-ended AgAuNPs. These NPs also showed antimicrobial and antibiofilm activity against E. coli, A. baumannii, S. aureus, and a mixed culture of A. baumannii and S. aureus. AgNPs inhibited biofilm in the range of 96%-99% and AgAuNPs from 93% to 98% in single-culture biofilms. AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus. AgNPs also showed good biofilm disruption, with the highest of 88% in A. baumannii. Conclusion: This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms.

10aAgAuNPs10aAgNPs10aAuNPs10abiofilm inhibition and disruption10aGC-TOF-MS10aP. zeylanica1 aSalunke, Gayatri, R.1 aGhosh, Sougata1 aKumar, R., J. Santosh1 aKhade, Samiksha1 aVashisth, Priya1 aKale, Trupti, S.1 aChopade, Snehal1 aPruthi, Vikas1 aKundu, Gopal1 aBellare, Jayesh, R.1 aChopade, Balu, Ananda uhttp://library.ncl.res.in/content/rapid-efficient-synthesis-and-characterization-silver-gold-and-bimetallic-nanoparticles-001776nas a2200241 4500008004100000022001400041245010600055210006900161260011000230300001400340490000700354520084200361100002601203700001901229700001901248700001701267700002401284700002601308700002301334700002601357700002501383856012601408 2015 eng d a1144-054600aSynthesis, DNA interaction and anticancer activity of 2-anthryl substituted benzimidazole derivatives0 aSynthesis DNA interaction and anticancer activity of 2anthryl su aTHOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLANDbROYAL SOC CHEMISTRYcAPR a4882-48900 v393 a

2-Anthryl benzimidazole derivatives (5-7) with hydrogen, carboxyl and benzoyl substituents at the 5th position have been synthesized using a silica supported periodic acid catalyst. The DNA cleavage activity of 5-7 was studied in the presence of light using pBR322 plasmid DNA and was shown to vary with substitution at the 5th position of benzimidazole derivatives. DNA binding studies using ethidium bromide displacement assay demonstrated the non-intercalative binding mode of 5-7. The anticancer activity of these target molecules was tested against MCF-7 and HL-60 cell lines, and they exhibited remarkable activity in the micromolar range. Cellular uptake and morphological changes were confirmed by fluorescence and confocal microscopy. A molecular docking study was carried out to explore the DNA binding mechanism of 5-7.

1 aSontakke, Vyankat, A.1 aKate, Anup, N.1 aGhosh, Sougata1 aMore, Piyush1 aGonnade, Rajesh, G.1 aKumbhar, Navanath, M.1 aKumbhar, Anupa, A.1 aChopade, Balu, Ananda1 aShinde, Vaishali, S. uhttp://library.ncl.res.in/content/synthesis-dna-interaction-and-anticancer-activity-2-anthryl-substituted-benzimidazole-002340nas a2200289 4500008004100000022001400041245007400055210006900129260000900198300001100207490000700218520144200225653001601667653002601683653002301709653002101732653003201753100002101785700002101806700002301827700002001850700001901870700002001889700002201909700001901931856010001950 2021 eng d a1664-302X00aAntimicrobial synergy of silver-platinum nanohybrids with antibiotics0 aAntimicrobial synergy of silverplatinum nanohybrids with antibio cFEB  a6109680 v113 a

Various bacterial pathogens are responsible for nosocomial infections resulting in critical pathophysiological conditions, mortality, and morbidity. Most of the bacterial infections are associated with biofilm formation, which is resistant to the available antimicrobial drugs. As a result, novel bactericidal agents need to be fabricated, which can effectively combat the biofilm-associated bacterial infections. Herein, for the first time we report the antimicrobial and antibiofilm properties of silver-platinum nanohybrids (AgPtNHs), silver nanoparticles (AgNPs), and platinum nanoparticles (PtNPs) against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The AgPtNHs were synthesized by a green route using Dioscorea bulbifera tuber extract at 100 degrees C for 5 h. The AgPtNHs ranged in size from 20 to 80 nm, with an average of similar to 59 nm. AgNPs, PtNPs, and AgPtNHs showed a zeta potential of -14.46, -1.09, and -11.39 mV, respectively. High antimicrobial activity was observed against P. aeruginosa and S. aureus and AgPtNHs exhibited potent antimicrobial synergy in combination with antibiotics such as streptomycin, rifampicin, chloramphenicol, novobiocin, and ampicillin up to variable degrees. Interestingly, AgPtNHs could inhibit bacterial biofilm formation significantly. Hence, co-administration of AgPtNHs and antibiotics may serve as a powerful strategy to treat bacterial infections.

10aantibiofilm10aantimicrobial synergy10abiogenic synthesis10aCharacterization10asilver-platinum nanohybrids1 aRanpariya, Bansi1 aSalunke, Gayatri1 aKarmakar, Srikanta1 aBabiya, Kaushik1 aSutar, Santosh1 aKadoo, Narendra1 aKumbhakar, Pathik1 aGhosh, Sougata uhttp://library.ncl.res.in/content/antimicrobial-synergy-silver-platinum-nanohybrids-antibiotics