02032nas a2200253 4500008004100000022001400041245014000055210006900195260005700264300001400321490000700335520111700342653001301459653002001472653001401492653001801506653001701524653002301541100002901564700001601593700001501609700002401624856013001648 2012 eng d a1590-426100aCharacterization of a new bacillus cereus ATUAVP1846 strain producing penicillin V acylase, and optimization of fermentation parameters0 aCharacterization of a new bacillus cereus ATUAVP1846 strain prod a233 SPRING ST, NEW YORK, NY 10013 USAbSPRINGERcSEP a1287-12930 v623 a

Penicillin acylases are involved chiefly in the industrial production of semisynthetic penicillins, which remain the most widely used group of antibiotics. We have isolated a new bacterial strain ATUAVP1846 that produces penicillin V acylase (PVA). Phylogenetic analysis using 16S rRNA sequencing showed 99.37% homology with Bacillus cereus. Maximum PVA production was observed with B. cereus ATUAVP1846 at 30A degrees C, pH 7 after 24 h fermentation time under submerged conditions. Highest enzyme productivity was achieved using sucrose as carbon source, and tryptone and ammonium hydrogen phosphate as nitrogen sources. Minimal medium containing 0.4% glucose and 0.3% ammonium hydrogen phosphate was found to be optimal for maximum PVA production from B. cereus ATUAVP1846. The crude enzyme from B. cereus ATUAVP1846 was partially purified using ammonium sulfate fractionation and showed highest enzymatic activity in the hydrolysis of penicillin V at 40A degrees C and pH 6. The crude enzyme preparation also showed unique substrate specificity, preferring ampicillin and cephalexin over penicillin V.

10a16S rRNA10aBacillus cereus10aisolation10aNtn hydrolase10aOptimization10aPenicillin acylase1 aSunder, Avinash, Vellore1 aKumar, Atul1 aNaik, Neha1 aPundle, Archana, V. uhttp://library.ncl.res.in/content/characterization-new-bacillus-cereus-atuavp1846-strain-producing-penicillin-v-acylase-and-002388nas a2200265 4500008004100000022001400041245007400055210006900129260007400198300001200272490000800284520150300292653001301795653001601808653002101824653001501845100002401860700002501884700002401909700002001933700001801953700002401971700002601995856010102021 2015 eng d a0003-607200aDeinococcus enclensis sp nov., isolated from a marine sediment sample0 aDeinococcus enclensis sp nov isolated from a marine sediment sam aVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDSbSPRINGERcJAN a141-1480 v1073 a

A novel pale-pink coloured strain, designated NIO-1023(T), was isolated from a marine sediment sample from Chorao Island, Goa, India. The taxonomic position of strain NIO-1023(T) was investigated by using a polyphasic approach. The cells were observed to be Gram-stain positive, coccal shaped and non-spore forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The strain NIO-1023(T) showed highest 16S rRNA gene sequence similarities with Deinococcus ficus (97.8 %), whereas other Deinococcus species showed less than 95 % sequence similarity. The DNA-DNA relatedness with respect to D. ficus CC-FR2-10(T) was 23.9 %. Chemotaxonomic data revealed that strain NIO-1023(T) contains only menaquinone MK-8 as the respiratory quinone and a complex polar lipid profile consisting of different unidentified glycolipids and polar lipids, two unknown phospholipids and three unknown phosphoglycolipids. As in other deinococci, one of these phosphoglycolipids was predominant in the profile. The predominant fatty acids were identified as C-17:1 w8c, C-16:1 w6c/w7c, C-15:1 w6c and C-17:1 w9c. The genomic DNA G + C content of strain NIO-1023(T) was determined to be 67.2 mol%. The biochemical and chemotaxonomic properties demonstrate that strain NIO-1023(T) represents a novel species, for which the name Deinococcus enclensis sp. nov. is proposed. The type strain is NIO-1023(T) (=DSM 25127(T) = NCIM 5456(T)).

10a16S rRNA10aDeinococcus10aMarine sediments10aPolyphasic1 aThorat, Meghana, N.1 aMawlankar, Rahul, R.1 aSonalkar, Vidya, V.1 aRamana, Venkata1 aJoseph, Neeta1 aShouche, Yogesh, S.1 aDastager, Syed, Gulam uhttp://library.ncl.res.in/content/deinococcus-enclensis-sp-nov-isolated-marine-sediment-sample-002586nas a2200301 4500008004100000022001400041245014900055210006900204260000800273300001200281490000800293520153000301653001301831653002901844653001401873653002901887100001801916700002201934700001401956700002001970700002001990700002702010700002602037700002902063700002402092700003002116856013802146 2017 eng d a0723-202000aInvestigation into the taxonomy of “ B. aminovorans” and its reclassification to the genus Domibacillus as Domibacillus aminovorans sp. nov.0 aInvestigation into the taxonomy of B aminovorans and its reclass cAUG a458-4670 v40 3 aThe species "Bacillus aminovorans" was categorized as species Incertae Sedis meaning taxa with uncertain taxonomic position because of insufficient description of phenotypic properties and lack of understanding of its phylogenetic relationship (Claus and Berkeley [5]). In this study two strains "B. aminovorans" DSM 1314T and DSM 4337 were phenotypically and phylogenomically analyzed. The strains are Gram-staining-positive, spore forming rods and unable to utilize/ferment most of the sugars tested except glucose. The major fatty acids are anteiso-C15:0 and iso-C15:0. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol and an aminophosphoglycolipid. The cell wall peptidoglycan is of A1γ type with meso-Dpm as the diamino acid. The menaquinone type present is MK-6. The G+C content of the genomic DNA of the type strain is 40.8mol%. These characteristics were found to be in line with description of the genus Domibacillus. Moreover phylogenetic analysis based on 16S rRNA gene sequence retrieved "B. aminovorans" within the genus Domibacillus with D. antri XD 80T as the closest relative. Additionally genome sequencing of the strains and their comparison with whole genome sequences of other Domibacillus spp. confirmed their distinctiveness and separate species status within the genus based on parameters of genome to genome distance calculator (GGDC) and average nucleotide identity (ANI) values. Therefore a novel species Domibacillus aminovorans sp. nov. (DSM 1314T =LMG 16796T) is proposed.10a16S rRNA10aDomibacillus aminovorans10aMALDI-TOF10a``Bacillus aminovorans''1 aVerma, Ashish1 aOjha, Anup, Kumar1 aPal, Yash1 aKumari, Parveen1 aSchumann, Peter1 aGruber-Vodicka, Harald1 aDastager, Syed, Gulam1 aNatarajan, Ramesh, Kumar1 aMayilraj, Shanmugam1 aKrishnamurthi, Srinivasan uhttp://library.ncl.res.in/content/investigation-taxonomy-%E2%80%9C-b-aminovorans%E2%80%9D-and-its-reclassification-genus-domibacillus02971nas a2200337 4500008004100000022001400041245022400055210006900279260000800348300001200356490000700368520179200375653001302167653001302180653003002193653001602223653001702239653001902256100001802275700001402293700002202307700001902329700001702348700002002365700002002385700002302405700002402428700002802452700003002480856012302510 2019 eng d a0723-202000aTaxonomic insights into the phylogeny of Bacillus badius and proposal for its reclassification to the genus Pseudobacillus as Pseudobacillus badius comb. nov. and reclassification of Bacillus wudalianchiensis Liu et al.0 aTaxonomic insights into the phylogeny of Bacillus badius and pro cMAY a360-3720 v423 a

The species Bacillus badius is one of the oldest members of the genus Bacillus isolated from faeces of children and was classified based on its ability to form endospores [8]. In 16S rRNA gene sequence and phylogenetic analysis, Bacillus badius DSM 23(T) shared low similarity (93.0%) and distant relationship with B. subtilis, the type species of the genus Bacillus indicating that it does not belong to this genus. Additional strains of the species, B. badius DSM 5610, DSM 30822 and B. encimensis SGD-V-25 (which has been recently reclassified as a member of this species) were included in the study to consider intraspecies diversity. Detailed molecular phylogenetic and comparative genome analysis clearly showed that the strains of B. badius were consistently retrieved outside the cluster of Bacillus sensu stricto and also distantly related to the genera Domibacillus and Quasibacillus. Further, the data from biochemical reactions (inability to ferment most carbohydrates), polar lipids profile (presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an aminophosphoglycolipid) and fatty acids supported the molecular analysis. Thus the four B. badius strains; DSM 23(T), DSM 5610, DSM 30822 and SGD-V-25 displayed sufficient demarcating phenotypic characteristics that warrant their classification as members of a novel genus and single species, for which the name Pseudobacillus badius gen. nov. comb. nov. is proposed with Pseudobacillus badius DSM 23(T) (= ATCC 14574(T)) as the type strain. Additionally, based on our findings from phenotypic, chemotaxonomic and genotypic parameters, Bacillus wudalianchiensis DSM 100757(T) was reclassified as Pseudobacillus wudalianchiensis comb. nov. (C) 2019 Elsevier GmbH. All rights reserved.

10a16S rRNA10aBacillus10aBacillus wudalianchiensis10aFatty acids10apolar lipids10aPseudobacillus1 aVerma, Ashish1 aPal, Yash1 aOjha, Anup, Kumar1 aKumari, Munesh1 aKhatri, Indu1 aRameshkumar, N.1 aSchumann, Peter1 aDastager, Syed, G.1 aMayilraj, Shanmugam1 aSubramanian, Srikrishna1 aKrishnamurthi, Srinivasan uhttp://library.ncl.res.in/content/taxonomic-insights-phylogeny-bacillus-badius-and-proposal-its-reclassification-genus