02372nas a2200277 4500008004100000022001400041245014600055210006900201260007100270300001600341490000600357520136000363653001301723653002501736653001301761653001201774653002301786653001801809100002901827700002601856700001901882700001701901700002701918700002201945856012701967 2014 eng d a1944-824400aSol-gel assisted fabrication of collagen hydrolysate composite scaffold: a novel therapeutic alternative to the traditional collagen scaffold0 aSolgel assisted fabrication of collagen hydrolysate composite sc a1155 16TH ST, NW, WASHINGTON, DC 20036 USAbAMER CHEMICAL SOCcSEP a15015-150250 v63 a

Collagen is one of the most widely used biomaterial for various biomedical applications. In this Research Article, we present a novel approach of using collagen hydrolysate, smaller fragments of collagen, as an alternative to traditionally used collagen scaffold. Collagen hydrolysate composite scaffold (CHCS) was fabricated with sol-gel transition procedure using tetraethoxysilane as the silica precursor. CHCS exhibits porous morphology with pore sizes varying between 380 and 780 mu m. Incorporation of silica conferred CHCS with controlled biodegradation and better water uptake capacity. Notably, 3T3 fibroblast proliferation was seen to be significantly better under CHCS treatment when compared to treatment with collagen scaffold. Additionally, CHCS showed excellent antimicrobial activity against the wound pathogens Staphylococcus aureus, Bacillus subtilis, and Escherichia coli due to the inherited antimicrobial activity of collagen hydrolysate. In vivo wound healing experiments with full thickness excision wounds in rat model demonstrated that wounds treated with CHCS showed accelerated healing when compared to wounds treated with collagen scaffold. These findings indicate that the CHCS scaffold from collagen fragments would be an effective and affordable alternative to the traditionally used collagen structural biomaterials.

10acollagen10acollagen hydrolysate10ascaffold10asol-gel10atissue engineering10awound Healing1 aRamadass, Satiesh, Kumar1 aPerumal, Sathiamurthi1 aGopinath, Arun1 aNisal, Anuya1 aSubramanian, Saravanan1 aMadhan, Balaraman uhttp://library.ncl.res.in/content/sol-gel-assisted-fabrication-collagen-hydrolysate-composite-scaffold-novel-therapeutic-002209nas a2200277 4500008004100000022001300041245014900054210006900203260000900272300001600281490000600297520121800303653003101521653003401552653001801586653003701604653001301641653001301654653002201667653002201689653001501711653002301726100002201749700003101771856012901802 2018 eng d a2470134300aSurface modification of polymers for tissue engineering applications: arginine acts as a sticky protein equivalent for viable cell accommodation0 aSurface modification of polymers for tissue engineering applicat cAPR  a4242–42510 v33 a

Hydrophobic polymers, for their favorable mechanical properties, are a popular choice as permanent bioimplants. These materials remain absolutely bioinert for years, but throw up challenges when it comes to fast integration with healthy tissue. Addressing this, herein, we present a surface-modification technique of converting the hydrophobic surface of a polymeric film into a hydrophilic one using a layer-by-layer assembly process involving gold nanoparticles and small molecules like amino acids. These films showed much improved animal cell (murine fibroblast) adherence properties compared to commercially available tissue culture plates. Moreover, arginine-modified films exhibited a nearly equivalent cell viability compared to the films modified with the natural extracellular matrix component fibronectin. The surface hydrophilicity and roughness of our novel film were characterized by contact angle measurement and atomic force microscopy. Cell counting, fluorescence microscopy, cell viability, and collagen estimation assay were employed to demonstrate that our film favored a much improved cell adherence, and accommodation in comparison to the commercially available tissue culture plates.

10aCell and Molecular biology10aExtracellular matrix proteins10aNanoparticles10aPhysical and chemical properties10aPolymers10aProteins10aSurface structure10aSurface treatment10aThin films10atissue engineering1 aSengupta, Poulomi1 aPrasad, Bhagavatula, L. V. uhttp://library.ncl.res.in/content/surface-modification-polymers-tissue-engineering-applications-arginine-acts-sticky-protein03030nas a2200289 4500008004100000022001400041245012300055210006900178260000800247300001100255490000800266520207100274653001802345653002302363653001502386653002002401653001402421653002302435100002502458700001702483700002202500700001902522700002202541700002802563700001802591856013102609 2020 eng d a0928-493100aPoly (vinylpyrrolidone)-iodine engineered poly (epsilon-caprolactone) nanofibers as potential wound dressing materials0 aPoly vinylpyrrolidoneiodine engineered poly epsiloncaprolactone  cMAY a1107310 v1103 a

Facilitating the process of wound healing and effective treatment of wounds remains a serious challenge in healthcare. Wound dressing materials play a major role in the protection of wounds and in accelerating the natural healing process. In the present study, novel core/shell (c/s) nanofibrous mats of poly(vinyl pyrrolidone)-iodine (PVPI) and polycaprolactone (PCL) were fabricated using a co-axial electrospinning process followed by their surface modification with poly-L-lysine. The developed nanofibrous mats were extensively characterized for their physicochemical properties using various analytical techniques. The core/shell structure of the PVP-I/PCL nanofibers was confirmed using TEM analysis. The PVP-I release studies showed an initial burst phase followed by a sustained release pattern of PVP-I over a period of 30 days. The developed nanofibers exhibited higher BSA and fibrinogen adsorption as compared to pristine PCL. Cytotoxicity studies using MTT assay demonstrated that the PVP-I/PCL (c/s) nanofibers were cytocompatible at optimized PVP-I concentration (3 wt%). The PCL-poly-L-lysine and PVP-I/PCL-poly-L-lysine nanofibers exhibited higher cell viability (24.2% and 21.4% higher at day 7) when compared to uncoated PCL and PVP-I/PCL nanofibers. The PVP-I/PCL nanofibers showed excellent antimicrobial activity against both Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria. The inflammatory response of Mouse RAW 264.7 macrophage cells towards the nanofibers was studied using RTPCR. It revealed that the pro-inflammatory cytokines (TNF-alpha and IL-1 beta) were significantly upregulated on PCL nanofibers, while their expression was comparatively lower on poly-L-lysine coated PCL or PVP-I/PCL(c/s) nanofibers. Overall, the study highlights the ability of poly-L-lysine coated PVP-I/PCL (c/s) nanofibers as potential wound dressing materials effectively facilitating the early stage wound healing and repair process by virtue of their selective modulation of inflammation, cell adhesion and antimicrobial properties.

10abiodegradable10aControlled release10aCore/shell10aelectrospinning10ascaffolds10atissue engineering1 aShitole, Ajinkya, A.1 aRaut, Piyush1 aGiram, Prabhanjan1 aRade, Priyanka1 aKhandwekar, Anand1 aGarnaik, Baijayantimala1 aSharma, Neeti uhttp://library.ncl.res.in/content/poly-vinylpyrrolidone-iodine-engineered-poly-epsilon-caprolactone-nanofibers-potential-wound02660nas a2200241 4500008004100000022001400041245013400055210006900189260000800258300001100266490000700277520181500284653001902099653001302118653001502131653002302146653001802169100002902187700002102216700001902237700003402256856012802290 2022 eng d a1773-224700aBioinspired hyaluronic acid based nanofibers immobilized with 3, 4-difluorobenzylidene curcumin for treating bacterial infections0 aBioinspired hyaluronic acid based nanofibers immobilized with 3  cAUG a1034800 v743 a

Curcumin (Cur) is a natural polyphenol with multifaceted pharmacological functions, exploited extensively for biomedical applications. Traditionally curcumin is being used as an antimicrobial agent. However, to improvise the pharmacological properties, it is being modified synthetically. One of such modified Cur is 3, 4- difluorobenzylidene curcumin (CDF) which is aimed for enhancing the anti-cancer properties. Though there are reports on the studies of anti-cancer properties involving CDF, the anti-bacterial property is yet to be demonstrated. Accordingly, in our studies, we prepared bioinspired hyaluronic acid blends immobilized with CDF and fabricated non-woven nanofiber mats. These nanofiber mats were characterized and demonstrated in vitro cell culture studies, which involved cell viability, hemolysis, anti-bacterial and cell scratch assay to understand their efficacy in treating bacteria. The molecular docking studies of CDF and Cur were performed on the dihydrofolate reductase (DHFR) enzyme receptor, which is an essential protein of S.auerus (Staphylococcus aureus). The results of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay, and hemolysis of the respective nanofiber mats with Cur and CDF showed non-toxicity and were compatible with blood cells. Further, the cell proliferation and adherence recorded >60% fibroblast cells for the nanofiber mats. The anti-bacterial property of Cur and CDF was similar. The in vitro release studies for the respective Cur and CDF loaded nanofiber mats recorded a release of 25 and 37%, respectively. From these studies, we concluded that the CDF sustained its antibacterial property in addition to the improved anti-cancer property; hence CDF being synergetic, it will have a better scope in cancer therapy.

10aanti-bacterial10acurcumin10ananofibers10atissue engineering10awound Healing1 aPatel, Pratikshkumar, R.1 aSingam, Amarnath1 aIyer, Arun, K.1 aGundloori, Rathna, Venkata Na uhttp://library.ncl.res.in/content/bioinspired-hyaluronic-acid-based-nanofibers-immobilized-3-4-difluorobenzylidene-curcumin01993nas a2200217 4500008004100000022001400041245007400055210006900129260000800198300001000206490000700216520129700223653001601520653001801536653002001554653001401574653002301588100002901611700003401640856010101674 2023 eng d a1042-714700aReview on electrospun nanofibers for multiple biomedical applications0 aReview on electrospun nanofibers for multiple biomedical applica cJAN a44-630 v343 a

Electrospinning is a well-known technique since 1544 to fabricate nanofibers using different materials like polymers, metals oxides, proteins, and many more. In recent years, electrospinning has become the most popular technique for manufacturing nanofibers due to its ease of use and economic viability. Nanofibers have remarkable properties like high surface-to-volume ratio, variable pore size distribution (10-100 nm), high porosity, low density, and are suitable for surface functionalization. Therefore, electrospun nanofibers have been utilized for numerous applications in the pharmaceutical and biomedical field like tissue engineering, scaffolds, grafts, drug delivery, and so on. In this review article, we will be focusing on the versatility, current scenario, and future endeavors of electrospun nanofibers for various biomedical applications. This review discusses the properties of nanofibers, the background of the electrospinning technique, and its emergence in chronological order. It also covers the various types of electrospinning methods and their mechanism, further elaborating the factors affecting the properties of nanofibers, and applications in tissue engineering, drug delivery, nanofibers as biosensor, skin cancer treatment, and magnetic nanofibers.

10abiomaterial10adrug delivery10aelectrospinning10aNanofiber10atissue engineering1 aPatel, Pratikshkumar, R.1 aGundloori, Rathna, Venkata Na uhttp://library.ncl.res.in/content/review-electrospun-nanofibers-multiple-biomedical-applications