02254nas a2200265   4500008004100000022001400041245013400055210006900189260009500258300001200353490000800365520121200373653002101585653002401606653003501630653001901665653002301684100002501707700002401732700002801756700002501784700002601809700003001835856012301865       2011                        eng d  a0003-269700aCarbon nanotube-modified sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weight determination of proteins0 aCarbon nanotubemodified sodium dodecyl sulfatepolyacrylamide gel  a525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USAbACADEMIC PRESS INC ELSEVIER SCIENCEcFEB  a230-2350 v4093 a<p>The effect of incorporating carbon nanotubes (CNTs) in the gel matrix on the electrophoretic mobility of proteins based on their molecular weight differences was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). More specifically, a reduction in standard deviation in the molecular weight calibration plots by 55% in the case of multiwalled carbon nanotubes (MWCNTs) and by 34% in the case of single-walled carbon nanotubes (SWCNTs) compared with that of pristine polyacrylamide gels was achieved after incorporating an insignificant amount of functionalized CNTs into the gel matrix. A mechanism based on a more uniform pore size distribution in CNT modified polyacrylamide gel matrix is proposed. Furthermore, the impact of SWCNTs and MWCNTs on the mobility of proteins in different molecular weight regimes at a given acrylamide concentration offers a tunable gel matrix in terms of the selection of molecular weight ranges of proteins. The robustness and excellent reproducibility of the CNT-PAGE protocol are expected to have a significant impact on the molecular weight determination of newly isolated proteins. (C) 2010 Elsevier Inc. All rights reserved.</p>10aCarbon nanotubes10aGel electrophoresis10aMolecular weight determination10aPolyacrylamide10aProtein separation1 aParthasarathy, Meera1 aDebgupta, Joyashish1 aKakade, Bhalchandra, A.1 aAnsary, Abu, Ayoobul1 aKhan, Mohammad, Islam1 aPillai, Vijayamohanan, K.  uhttp://library.ncl.res.in/content/carbon-nanotube-modified-sodium-dodecyl-sulfate-polyacrylamide-gel-electrophoresis-002337nas a2200229   4500008004100000022001400041245016100055210006900216260009900285300001400384490000700398520140000405653002401805653001901829653002001848100002301868700002301891700001901914700002501933700002801958856012101986       2013                        eng d  a0032-386100aThermodynamic behavior of hydrophobically modified polyacrylamide containing random distribution of hydrophobes: experimental and theoretical investigations0 aThermodynamic behavior of hydrophobically modified polyacrylamid  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAY  a2676-26890 v543 a<p>Two series of Hydrophobically Modified Polyacrylamides (HMPAMs) were prepared by free radical copolymerization of trimethylsilylacrylamide with 3-pentadecylcyclohexylacrylamide (PDCAM) or dodecylacrylamide (DDAM) followed by removal of trimethylsilyl protecting groups. The solution properties of HMPAMs were studied in dilute aqueous solution by fluorescence spectroscopy, NMR and viscometry. The properties in aqueous solution of HMPAMs clearly displayed the formation of hydrophobic interactions between alkyl stickers. While dodecyl containing HMPAMs mainly showed a progressive collapse of the copolymer coil with the increasing fraction of hydrophobic moieties (weak intra-chain associations), pentadecylcyclohexyl groups containing HMPAMs exhibited a much lower solubility in water due to strong intra- and inter-chain associations. Experimental data were analyzed using the mean field theory developed by Semenov and Rubinstein (SR) for associating polymers. Using a set of realistic parameters, taking into account the solubility of the backbone, the fraction of stickers and the strength of hydrophobic attractions, we show that the SR model offers a quantitative description of the thermodynamic properties of HMPAMs in terms of individual and collective behavior of polymers chains; i.e. swelling and phase separation, respectively. (C) 2013 Elsevier Ltd. All rights reserved.</p>10aAssociating polymer10aPolyacrylamide10aSelf-assembling1 aHourdet, Dominique1 aDucouret, Guylaine1 aVarghese, Sony1 aBadiger, Manohar, V.1 aWadgaonkar, Prakash, P.  uhttp://library.ncl.res.in/content/thermodynamic-behavior-hydrophobically-modified-polyacrylamide-containing-random-0