02285nas a2200277 4500008004100000022001400041245010500055210006900160260007600229300001000305490000800315520124200323653003001565653003901595653000901634653004401643653000801687653004701695100002601742700001601768700002701784700002101811700002101832700002801853856012601881 2012 eng d a0179-954100aMapping of tms8 gene for temperature-sensitive genic male sterility (TGMS) in rice (Oryza sativa L.)0 aMapping of tms8 gene for temperaturesensitive genic male sterili aCOMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USAbWILEY-BLACKWELLcFEB a42-470 v1313 a

Genetic analysis of F-2 and backcross populations of an induced temperature-sensitive genic male sterility (TGMS) mutant source F 61 with normal pollen parents revealed that TGMS trait was controlled by a single recessive gene. Molecular tagging of TGMS trait was attempted using polymorphic randomly amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers through bulked segregant analysis. The RAPD primers UBC 345830, UBC 313927, microsatellites RM224 and RM21 produced putative markers, which differentiate parents and bulks from sterile parent and sterile bulks. The RAPD analysis of individual F-2 plants with the primer UBC345(830) showed perfect marker-phenotype cosegregation. The 830-bp RAPD fragment, which segregated with TGMS locus at a distance of 1.33 cM, was eluted and cloned, and sequence information was used for designing sequence-characterized amplified region (SCAR) primer, which cosegregated with TGMS locus at a distance of 0.8 cM. TGMS locus was mapped onto chromosome 11 using RM21 and RM224, flanking it at a distance of 4.3 and 3.0 cM, respectively. The DNA markers tightly linked to TGMS gene (tms8) in F 61 can be cost effectively used for marker-assisted selection of TGMS trait.

10aBulked segregant analysis10arandomly amplified polymorphic DNA10arice10asequence-characterized amplified region10aSSR10atemperature-sensitive genic male sterility1 aHussain, Appibhai, J.1 aAli, Jauhar1 aSiddiq, Ebrahimali, A.1 aGupta, Vidya, S.1 aReddy, Umesh, K.1 aRanjekar, Prabhakar, K. uhttp://library.ncl.res.in/content/mapping-tms8-gene-temperature-sensitive-genic-male-sterility-tgms-rice-oryza-sativa-l-002725nas a2200277 4500008004100000022001400041245011000055210006900165260000800234300001100242490000800253520182900261653001502090653002402105653001502129653001702144653001602161653000902177100002102186700002502207700001702232700002302249700002302272700002702295856012502322 2022 eng d a0944-501300aExploring the core microbiota in scented rice (Oryza sativa L.) rhizosphere through metagenomics approach0 aExploring the core microbiota in scented rice Oryza sativa L rhi cOCT a1271570 v2633 a

Rice is a major food crop cultivated around the globe. Specially scented rice varieties are of commercial importance but they are low-yielding. The rhizospheric microflora plays a significant role in improving yield and aroma. However, the core microbiome of the scented rice rhizosphere is comparatively less explored. Here, we analyzed the core microbiome associated with the rhizosphere of the scented (Ambemohar-157 and Dehradun basmati) in comparison with non-scented rice (Kolam and Arize 6444 Gold) cultivated at two different geoclimatic zones of India (Maharashtra and Uttarakhand) using the metagenomics approach. The alpha and beta diversity analysis showed that the microbial communities associated with scented and non-scented varieties significantly changes with respect to richness, diversity, and evenness. The taxonomic profiling revealed the variation in composition, diversity, and abundance of the microbiome in terms of phyla and genera associated with scented rice varieties over non-scented. The cluster analysis distinguishes the microbial communities based on their geographical positions. The core microbiome analysis revealed that scented rice rhizosphere shelters distinct and unique microbiota. 28.6 % of genera were exclusively present only in the scented rice rhizosphere. The putative functional gene annotation revealed the high abundance of genes related to the biosynthesis of 2-acetyl-1-pyrroline (2AP) precursors in scented rice. The precursor feeding analysis revealed proline as a preferred substrate by 2AP synthesizing bacteria. The 2AP precursor proline and proline metabolism genes showed a positive correlation. The scented rice-specific rhizobacteria pointed out in this study can be used as bioinoculants for enhancing aroma, yield, and sustainable rice cultivation.

10aMetagenome10aMicrobial community10aMicrobiome10aOryza sativa10aRhizosphere10arice1 aDhondge, Harshal1 aBarvkar, Vitthal, T.1 aPaul, Dhiraj1 aDastager, Syed, G.1 aPable, Anupama, A.1 aNadaf, Altafhusain, B. uhttp://library.ncl.res.in/content/exploring-core-microbiota-scented-rice-oryza-sativa-l-rhizosphere-through-metagenomics02948nas a2200385 4500008004100000022001400041245017500055210006900230260000800299300001200307490000800319520168600327653001902013653001802032653001502050653001402065653001402079653002202093653001402115653000902129653001202138653001602150100003302166700002002199700002302219700002902242700002002271700003302291700002502324700002502349700002102374700001902395700002202414856012602436 2022 eng d a0981-942800aStacking herbicide detoxification and resistant genes improves glyphosate tolerance and reduces phytotoxicity in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.)0 aStacking herbicide detoxification and resistant genes improves g cOCT a126-1380 v1893 a

Glyphosate residues retained in the growing meristematic tissues or in grains of glyphosate-resistant crops affect the plants physiological functions and crop yield. Removing glyphosate residues in the plants is desirable with no penalty on crop yield and quality. We report a new combination of scientific strategy to detoxify glyphosate that reduces the residual levels and improve crop resistance. The glyphosate detoxifying enzymes Aldo-keto reductase (AKR1) and mutated glycine oxidase (mGO) with different modes of action were co-expressed with modified EPSPS, which is insensitive to glyphosate in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.). The transgenic tobacco plants expressing individual PsAKR1, mGO, CP4-EPSPS, combinations of PsAKR1:CP4EPSPS, PsAKR1:mGO, and multigene with PsAKR1: mGO: CP4EPSPS genes were developed. The bio-efficacy studies of in-vitro leaf regeneration on different concentrations of glyphosate, seedling bioassay, and spray on transgenic tobacco plants demonstrate that glyphosate detoxification with enhanced resistance. Comparative analysis of the transgenic tobacco plants reveals that double and multigene expressing transgenics had reduced accumulation of shikimic acid, glyphosate, and its primary residue AMPA, and increased levels of sarcosine were observed in all PsAKR1 expressing transgenics. The multigene expressing rice transgenics showed improved glyphosate resis-tance with yield maintenance. In summary, results suggest that stacking genes with two different detoxification mechanisms and insensitive EPSPS is a potential approach for developing glyphosate-resistant plants with less residual content.

10aDetoxification10aGene stacking10aGlyphosate10aHerbicide10aMultigene10aResidual toxicity10aResistant10arice10aTobacco10aTransgenics1 aVennapusa, Amaranatha, Reddy1 aAgarwal, Subham1 aHm, Hanumanth, Rao1 aAarthy, Thiagarayaselvam1 aBabitha, K., C.1 aThulasiram, Hirekodathakallu1 aKulkarni, Mahesh, J.1 aMelmaiee, Kalpalatha1 aSudhakar, Chinta1 aUdayakumar, M.1 aVemanna, Ramu, S. uhttp://library.ncl.res.in/content/stacking-herbicide-detoxification-and-resistant-genes-improves-glyphosate-tolerance-and