02772nas a2200301 4500008004100000022001400041245006400055210006400119260009900183300000900282490000700291520177900298653002802077653001402105653002302119653002502142653002902167653002202196653001302218100002202231700001802253700001502271700001402286700003002300700002002330700002302350856009702373 2005 eng d a0144-861700aEnzymatic hydrolysis of delignified bagasse polysaccharides0 aEnzymatic hydrolysis of delignified bagasse polysaccharides aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcOCT a6-100 v623 a

Sugarcane bagasse, consisting of cellulose, xylan, and lignin, was chemically treated to generate bagasse samples with continuously decreasing content of lignin. These bagasse samples were hydrolyzed by cellulase and xylanase enzymes, produced earlier by Penicillium janthinellum NCIM 1171 in the same bagasse polysaccharides production medium. The hydrolysis was carried out by using different concentrations of the enzymes at two different temperatures, 30 and 50 degrees C, taking hydrolysis of Avicel as control. It was found that while the maximum hydrolysis for Avicel was 70% that of some of the bagasse polysaccharides was as high as 95%. The products of hydrolysis were glucose, xylose, and arabinose, as confirmed by high pressure ion chromatography (HPIC). It is interesting to note that arabinose, which constitutes about 10% of the weight of bagasse xylan, could also be released easily by the enzymes. Also, the initial rates of hydrolysis was found to be much higher for the bagasse polysaccharides, and in some cases about 90% of the hydrolysis occurred within 20 h. Amongst all bagasse samples, the sample with (Kappa no. 1.2, lignin content 0.18%) gave the highest degree of hydrolysis at 50 degrees C. Even the bagasse polysaccharide with Kappa no. 16.8 (lignin content 2.5%) underwent greater extent of hydrolysis than Avicel. Apparently, the delignified bagasse medium appears to be a facile medium for the combined hydrolytic action of the cellulase and xylanase enzymes. Considering that sugarcane bagasse is a waste biomass material available in abundance annually, this methodology can be used to value-add to this biomass to produce sugars, which can be fermented to produce biofuels like ethanol. (C) 2005 Elsevier Ltd. All rights reserved.

10abagasse polysaccharides10aCellulase10achemical treatment10aEnzymatic hydrolysis10aPenicillium janthinellum10aSugarcane bagasse10aXylanase1 aAdsul, Mukund, G.1 aGhule, J., E.1 aShaikh, H.1 aSingh, R.1 aBastawade, Kulbhushan, B.1 aGokhale, D., V.1 aVarma, Anjanikumar uhttp://library.ncl.res.in/content/enzymatic-hydrolysis-delignified-bagasse-polysaccharides-002505nas a2200301 4500008004100000022001400041245010600055210006900161260009900230300001000329490000700339520143700346653002101783653002101804653003201825653002101857653002201878653002401900653002001924100001401944700001401958700002101972700002001993700003002013700002002043700002302063856011702086 2005 eng d a0144-861700aLignin-carbohydrate complexes from sugarcane bagasse: preparation, purification, and characterization0 aLignincarbohydrate complexes from sugarcane bagasse preparation  aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcOCT a57-660 v623 a

Lignin-carbohydrate complexes were isolated from sugarcane bagasse by a process, which yielded sulfur-free lignins. These could be made carbohydrate-free, if necessary, by treatment with xylanase enzyme. A study of the preparation, purification, and characterization of such lignin-carbohydrate complexes, comparison with commercial lignin samples (wood based as well as bagasse based) and some other lignin derivatives was made by using a variety of analytical tools such as FTIR, HPLC at three different UV-wavelengths, GPC, thermal analysis and elemental analysis. The use of such a diverse range of lignin-carbohydrate complex samples enabled us to predict the sensitivity of the various analytical techniques for characterization of complex polymers containing carbohydrate moieties. Evidence for lignin-carbohydrate complex was detectable by FTIR as well as HPLC studies. Thermal analysis studies showed the crucial effect of carbohydrate groups, the content of aliphatic chains, and the sulfur content of the lignins. Generalized structures of lignin-carbohydrate complexes obtained from various sources using different preparation methods and chemical modifications are presented. This will aid the applications development effort with advantageously using lignins containing low levels of carbohydrate moieties as reactive sites as well as biodegradability inducing sites. (C) 2005 Elsevier Ltd. All rights reserved.

10abiodegradability10ahardwood lignins10alignin-carbohydrate complex10asoftwood lignins10aSugarcane bagasse10asulfur-free lignins10axylanase enzyme1 aSingh, R.1 aSingh, S.1 aTrimukhe, K., D.1 aPandare, K., V.1 aBastawade, Kulbhushan, B.1 aGokhale, D., V.1 aVarma, Anjanikumar uhttp://library.ncl.res.in/content/lignin-carbohydrate-complexes-sugarcane-bagasse-preparation-purification-and-001876nas a2200241 4500008004100000022001400041245012800055210006900183260009900252300001400351490000800365520092800373653002601301653002501327653002601352653002601378653002201404100002001426700002301446700002501469700001401494856012601508 2009 eng d a0960-852400aHydrolysis of cellulose derived from steam exploded bagasse by penicillium cellulases: comparison with commercial cellulase0 aHydrolysis of cellulose derived from steam exploded bagasse by p aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcDEC a6679-66810 v1003 a

A complete cellulase from Penicillium pinophilum was evaluated for the hydrolysis of a-cellulose derived from steam exploded sugarcane bagasse and other cellulosic substrates. alpha-Cellulose at 1% substrate concentration was completely hydrolyzed by Penicillium cellulase within 3 h wherein at 10% the hydrolysis was 100% within 24 h with an enzyme loading of 10 FPU/g. The hydrolysate yielded glucose as major end product as analyzed by HPLC. Under similar conditions, hydrolysis of Sigmacell (microcrystalline cellulose), CP-123 (pulverized cellulose powder) and ball milled Solka Floc were 42%, 56% and 52%, respectively. Further the hydrolysis performance of Penicillium sp. cellulase is compared with Trichoderma reesei cellulase (Accellerase (TM) 1000) from Genencore. The kinetics of hydrolysis with respect to enzyme and substrate concentration will be presented. (C) 2009 Elsevier Ltd. All rights reserved.

10aAccellerase (TM) 100010aEnzymatic hydrolysis10aHigh beta-glucosidase10aPenicillium cellulase10aSugarcane bagasse1 aSingh, Rajkumar1 aVarma, Anjanikumar1 aLaxman, Ryali, Seeta1 aRao, Mala uhttp://library.ncl.res.in/content/hydrolysis-cellulose-derived-steam-exploded-bagasse-penicillium-cellulases-comparison-002096nas a2200253 4500008004100000022001400041245013200055210006900187260009900256300001000355490000700365520113700372653002201509653002201531653001801553653001601571653002201587653001801609100002201627700002301649700001901672700002301691856012801714 2009 eng d a0144-861700aUtilization of sugarcane bagasse cellulose for producing cellulose acetates: novel use of residual hemicellulose as plasticizer0 aUtilization of sugarcane bagasse cellulose for producing cellulo aTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLANDbELSEVIER SCI LTDcMAR a23-290 v763 a

Sugarcane bagasse was fractionated to cellulose, hemicellulose and lignin by a proprietary steam explosion process, followed by downstream purifications, developed in our laboratory. The fractionated cellulose contained similar to 94% cellulose, about similar to 5% hemicellulose, traces of lignin (similar to 0.2%), and similar to 1% ash. The cellulose was acetylated under heterogeneous conditions to obtain cellulose acetates. These were extensively characterized using FTIR, TGA, DSC, GPC, HPIC, WAXRD, and viscometry. The novel feature of this study was the utilization of the hemicellulose content (5%) of bagasse cellulose as an internal plasticizer. Through kinetic experimentation, we have demonstrated that the residual hemicellulose need not be considered as an impurity; rather it can be used in acetylated form as a plasticizer as well as a biodegradable additive for cellulose acetates made from slightly impure cellulose produced from non-wood origin. Our results therefore show how lignocellulosic agricultural wastes can be utilized to produce high value plastics. (C) 2008 Elsevier Ltd. All rights reserved.

10aBagasse cellulose10aCellulose acetate10aHemicellulose10aPlasticizer10aSugarcane bagasse10aXylan acetate1 aShaikh, Hamid, M.1 aPandare, Kiran, V.1 aNair, Greeshma1 aVarma, Anjanikumar uhttp://library.ncl.res.in/content/utilization-sugarcane-bagasse-cellulose-producing-cellulose-acetates-novel-use-residual-002177nas a2200241 4500008004100000022001400041245011600055210006900171260000800240300001200248490000700260520134600267653002401613653002401637653002901661653002201690100001701712700002101729700002001750700001601770700001901786856013001805 2024 eng d a0971-457X00aSynthetic layered double hydroxide on biodegradable support: an efficient adsorbent for defluoridation of water0 aSynthetic layered double hydroxide on biodegradable support an e cJAN a152-1610 v313 a

Fluoride concentrations in drinking water above permissible levels and incidences of fluorosis among people have been reported from many parts of the world including India. Low-cost and biodegradable adsorbents are the preferred choice for the removal of fluoride from an aqueous medium. In the present study, zinc-aluminium layered double hydroxide (LDH) supported on sugarcane bagasse (raw and acid-treated) has been synthesized, characterized, and investigated for the defluoridation of water. The SEM micrographs of the supported adsorbents show good dispersion of the LDH particles on the support material. The defluoridation capacity of the LDH is enhanced by supporting it on bagasse. The adsorption capacity of supported adsorbents increased by 3-fold than the unsupported LDH adsorbent. The adsorption data have been well fitted to the Freundlich isotherm model indicating physical and multi-layer adsorption. The maximum fluoride adsorption capacity has been found to be 8.85 mg/g with 76.3% fluoride removal when the initial fluoride concentration is 11-12 mg/L. The pseudo-second-order kinetic model has been found suitable to explain the fluoride adsorption kinetics on the supported LDH adsorbents. The present study reveals that the bagasse-supported LDH adsorbent has a high potential for defluoridation of water.

10aadsorption kinetics10aFluoride adsorption10aLayered double hydroxide10aSugarcane bagasse1 aMayadevi, S.1 aKirandas, M., D.1 aManilal, A., M.1 aRoshini, N.1 aMandal, Sujata uhttp://library.ncl.res.in/content/synthetic-layered-double-hydroxide-biodegradable-support-efficient-adsorbent-defluoridation