02301nas a2200241   4500008004100000022001400041245011100055210006900166260000800235520145800243653002301701653002101724653001301745653002201758653001901780100001701799700002301816700002101839700002301860700002401883700002101907856013101928       2019                        eng d  a0721-759500aWhole proteome analysis of GA(3) response at panicle stage in Grape (Vitis vinifera) CV. Thompson seedless0 aWhole proteome analysis of GA3 response at panicle stage in Grap  cOCT3 a<p>Rachis elongation is a crucial process in producing good quality table grapes. In compact clustered grape variety like Thompson Seedless, treating the flower panicles with a mild dose of GA(3) results in loose clusters. We sprayed GA(3) on Thompson Seedless panicles immediately after emergence, and the samples were collected at 6 h and 24 h after application. Whole proteome analysis revealed significant differential expression of 530 proteins of the total 1288 expressed proteins at two time-points. GO term enrichment analysis revealed enrichment of GO terms related to translation, biosynthetic processes, and photosynthesis in GA(3)-treated samples. As the process of rachis elongation requires enhanced carbon metabolism and accumulation of sugars through the expansion of phloem area, we attribute this to the overexpression of several proteins involved in these processes. Significantly highly expressed proteins also belonged to biological processes like the generation of precursor metabolites, cellular protein metabolic processes, response to abiotic stimulus, and protein metabolic processes. This study might be the first of its kind in deciphering the contribution of a different group of proteins during rachis elongation as an early response to GA(3) application in seedless grapes. These results provide quality information on various physiological and biochemical changes occurring during early stages of rachis elongation.</p>
10aBunch architecture10aGibberellic acid10aProteome10aRachis elongation10aVitis vinifera1 aMaske, Smita1 aUpadhyay, Anuradha1 aJogaiha, Satisha1 aPatil, Sucheta, S.1 aKadoo, Narendra, Y.1 aGupta, Vidya, S.  uhttp://library.ncl.res.in/content/whole-proteome-analysis-ga3-response-panicle-stage-grape-vitis-vinifera-cv-thompson-seedless02573nas a2200337   4500008004100000022001400041245017400055210006900229260000800298300001100306490000800317520145400325653002101779653001301800653002701813653001801840653002301858100001801881700001601899700002201915700001901937700001901956700002001975700001901995700002602014700002402040700001602064700001602080700001702096856012202113       2022                        eng d  a0925-521400aTranscriptional and translational perturbation in abiotic stress induced physiological activities and metabolic pathway networks in spongy tissue disorder of mango fruit0 aTranscriptional and translational perturbation in abiotic stress  cJUN  a1118800 v1883 a<p>
	Spongy tissue formation is economically the most detrimental but agriculturally less focused physiological disorder in mango. `Alphonso' cultivar is highly prone to oxidative stress induced spongy tissue disorder impacting biochemical and metabolic profile, thereby affecting pulp quality and nutritional value of the fruit. In the present study, comparative analysis of spongy and healthy mesocarp tissues of `Alphonso' mango by transcriptomics using Illumina sequencing and proteomics using LC-MS approaches, respectively identified and quantified many genes and proteins in the metabolic pathways responsible for the spongy tissue development. The table green and the mid ripe stages of `Alphonso' fruit ripening were evaluated by the transcriptomic study and outcomes were validated using proteomic investigations for all the four ripening stages. Colossal amount of data including 30,582 transcripts, 10,800 gene ontologies and 387 putative proteins was generated from this analysis. Current multi-omics exploration revealed the development of abiotic stress (mainly oxidative stress) induced perturbations in various metabolic pathways and their interconnections, leading to the spongy tissue formation in mango. This further unfolded the altered cell wall degradation, ethylene and flavonoid biosynthesis, fruit ripening and flavor formation, thus hampering the fruit specific characteristics in mango with spongy tissue disorder.</p>
10aOxidative stress10aProteome10aspongy tissue disorder10aTranscriptome10a` Alphonso ` mango1 aOak, Pranjali1 aJha, Vineet1 aDeshpande, Ashish1 aTanpure, Rahul1 aDawkar, Vishal1 aMundhe, Swapnil1 aGhuge, Sandeep1 aPrabhudesai, Shrikant1 aKrishanpal, Anamika1 aJere, Abhay1 aGiri, Ashok1 aGupta, Vidya  uhttp://library.ncl.res.in/content/transcriptional-and-translational-perturbation-abiotic-stress-induced-physiological