We present a polyfluorene appended with protected L-glutamic acid that exhibited a reversible alpha-helix/beta-sheet-like conformation and helical porous fibrous morphology mimicking the super-structure of proteins. The new homochiral polymer probe enabled efficient heterogeneous enantioselective separation and chiral sensing of a wide variety of substrates from their aqueous racemic mixture using an easy `Filter-and-Separate' method.

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